gal4 dna binding domain (TaKaRa)
Structured Review
![Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8646/pmc12948646/pmc12948646__MPP-27-e70202-g007.jpg)
Gal4 Dna Binding Domain, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal4+dna+binding+domain/pGBKT7+DNA-BD+Vector/pmc12948646-190-20-23
Average 99 stars, based on 4586 article reviews
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1) Product Images from "Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers"
Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers
Journal: Molecular Plant Pathology
doi: 10.1111/mpp.70202
Figure Legend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.
Techniques Used: Expressing, Binding Assay, Selection, Plasmid Preparation
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