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gal4 dna binding domain  (TaKaRa)


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    Structured Review

    TaKaRa gal4 dna binding domain
    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.
    Gal4 Dna Binding Domain, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gal4+dna+binding+domain/pGBKT7+DNA-BD+Vector/pmc12948646-190-20-23
    Average 99 stars, based on 4586 article reviews
    gal4 dna binding domain - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers"

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers

    Journal: Molecular Plant Pathology

    doi: 10.1111/mpp.70202

    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.
    Figure Legend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Techniques Used: Expressing, Binding Assay, Selection, Plasmid Preparation

    Related Articles

    Binding Assay:

    Article Title: Functional analysis of HpMYB72 showed its positive involvement in high-temperature resistance via repressing the expression of HpCYP73A
    Article Snippet: Global warming has led to occasional occurrence of extreme climates, especially summer high temperature, which constrains crops in growth and development.. Pitaya (Hylocereus polyrhizus) characterizes in excellent tolerance to high temperature and drought stresses.. Previously, a differentially expressed R2R3-MYB transcription factor gene, designated as HpMYB72, was identified in pitaya as exposure to high temperature.

    Article Title: PagUNE12 encodes a basic helix-loop-helix transcription factor that regulates the development of secondary vascular tissue in poplar.
    Article Snippet: Secondary growth in woody plants generates new cells and tissues via the activity of the vascular cambium and drives the radial expansion of stems and roots.. It is regulated by a series of endogenous factors, especially transcription factors.. Here, we cloned the basic helix–loop–helix (bHLH) transcription factor gene UNFERTILIZED EMBRYO SAC12 (UNE12) from poplar (Populus alba × Populus glandulosa Uyeki) and used biochemical, molecular, and cytological assays to investigate the biological functions and regulatory mechanism of PagUNE12.

    Article Title: Overexpression of PpGL2 from Prunus persica enhanced soybean drought tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Article Title: Overexpression of PpGL2 from Prunus persica Enhanced Soybean Drought Tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Article Title: OsMYB99 Modulates Grain Wax Biosynthesis and Redox Homeostasis to Control Head Rice Yield and Chalkiness.
    Article Snippet: .. Full- length OsMYB99 CDS and truncations (OsMYB991- 117aa, OsMYB991- 160aa and OsMYB991- 210aa) were PCR- amplified and ligated downstream to the GAL4 DNA binding domain in pGBKT7 vector (Clontech, USA) by homologous recombination. .. Constructs and empty vector control (BD) were transformed into the yeast strain Y2HGold and plated on synthetic dropout medium without tryptophan (SD/- T).

    Sequencing:

    Article Title: LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean
    Article Snippet: Subcellular localization of LRM3‐GFP was monitored by visualizing GFP fluorescence using a Nikon confocal microscope C2 (Japan) with excitation at 488 nm and emission at 495–540 nm (Liang et al ., ). .. The full‐length LRM3 coding sequence, fused to the GAL4 DNA‐binding domain in the pGBKT7 vector (Clontech), was employed as bait in a yeast two‐hybrid assay screen of a soybean cDNA library conducted in our laboratory. .. Yeast AH109 cells harbouring the bait vector BD‐LRM3 were subsequently transformed with the soybean cDNA library cloned into the prey vector pGADT7 (Clontech) and selected on yeast synthetic dropout medium lacking Trp, Leu, His and Ade, supplemented with 30 mM 3‐AT.

    Plasmid Preparation:

    Article Title: LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean
    Article Snippet: Subcellular localization of LRM3‐GFP was monitored by visualizing GFP fluorescence using a Nikon confocal microscope C2 (Japan) with excitation at 488 nm and emission at 495–540 nm (Liang et al ., ). .. The full‐length LRM3 coding sequence, fused to the GAL4 DNA‐binding domain in the pGBKT7 vector (Clontech), was employed as bait in a yeast two‐hybrid assay screen of a soybean cDNA library conducted in our laboratory. .. Yeast AH109 cells harbouring the bait vector BD‐LRM3 were subsequently transformed with the soybean cDNA library cloned into the prey vector pGADT7 (Clontech) and selected on yeast synthetic dropout medium lacking Trp, Leu, His and Ade, supplemented with 30 mM 3‐AT.

    Article Title: A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla
    Article Snippet: After incubation for 24 h, the transformed onion epidermal cells were stained with DAPI (100 ng/mL) and visualized under an inverted fluorescence microscope (Zeiss, Jena, Germany). .. The complete and various truncated versions of the CDS of BpbHLH1 were PCR amplified and fused in‐frame to the GAL4 DNA‐binding domain in the pGBKT7 vector to generate the pGBKT7‐ BpbHLH1 construct (Clontech laboratories, Inc. Mountain View, California, USA). ..

    Y2H Assay:

    Article Title: LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean
    Article Snippet: Subcellular localization of LRM3‐GFP was monitored by visualizing GFP fluorescence using a Nikon confocal microscope C2 (Japan) with excitation at 488 nm and emission at 495–540 nm (Liang et al ., ). .. The full‐length LRM3 coding sequence, fused to the GAL4 DNA‐binding domain in the pGBKT7 vector (Clontech), was employed as bait in a yeast two‐hybrid assay screen of a soybean cDNA library conducted in our laboratory. .. Yeast AH109 cells harbouring the bait vector BD‐LRM3 were subsequently transformed with the soybean cDNA library cloned into the prey vector pGADT7 (Clontech) and selected on yeast synthetic dropout medium lacking Trp, Leu, His and Ade, supplemented with 30 mM 3‐AT.

    cDNA Library Assay:

    Article Title: LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean
    Article Snippet: Subcellular localization of LRM3‐GFP was monitored by visualizing GFP fluorescence using a Nikon confocal microscope C2 (Japan) with excitation at 488 nm and emission at 495–540 nm (Liang et al ., ). .. The full‐length LRM3 coding sequence, fused to the GAL4 DNA‐binding domain in the pGBKT7 vector (Clontech), was employed as bait in a yeast two‐hybrid assay screen of a soybean cDNA library conducted in our laboratory. .. Yeast AH109 cells harbouring the bait vector BD‐LRM3 were subsequently transformed with the soybean cDNA library cloned into the prey vector pGADT7 (Clontech) and selected on yeast synthetic dropout medium lacking Trp, Leu, His and Ade, supplemented with 30 mM 3‐AT.

    Activation Assay:

    Article Title: PagUNE12 encodes a basic helix-loop-helix transcription factor that regulates the development of secondary vascular tissue in poplar.
    Article Snippet: Secondary growth in woody plants generates new cells and tissues via the activity of the vascular cambium and drives the radial expansion of stems and roots.. It is regulated by a series of endogenous factors, especially transcription factors.. Here, we cloned the basic helix–loop–helix (bHLH) transcription factor gene UNFERTILIZED EMBRYO SAC12 (UNE12) from poplar (Populus alba × Populus glandulosa Uyeki) and used biochemical, molecular, and cytological assays to investigate the biological functions and regulatory mechanism of PagUNE12.

    Article Title: Overexpression of PpGL2 from Prunus persica enhanced soybean drought tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Article Title: Overexpression of PpGL2 from Prunus persica Enhanced Soybean Drought Tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Amplification:

    Article Title: PagUNE12 encodes a basic helix-loop-helix transcription factor that regulates the development of secondary vascular tissue in poplar.
    Article Snippet: Secondary growth in woody plants generates new cells and tissues via the activity of the vascular cambium and drives the radial expansion of stems and roots.. It is regulated by a series of endogenous factors, especially transcription factors.. Here, we cloned the basic helix–loop–helix (bHLH) transcription factor gene UNFERTILIZED EMBRYO SAC12 (UNE12) from poplar (Populus alba × Populus glandulosa Uyeki) and used biochemical, molecular, and cytological assays to investigate the biological functions and regulatory mechanism of PagUNE12.

    Article Title: A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla
    Article Snippet: After incubation for 24 h, the transformed onion epidermal cells were stained with DAPI (100 ng/mL) and visualized under an inverted fluorescence microscope (Zeiss, Jena, Germany). .. The complete and various truncated versions of the CDS of BpbHLH1 were PCR amplified and fused in‐frame to the GAL4 DNA‐binding domain in the pGBKT7 vector to generate the pGBKT7‐ BpbHLH1 construct (Clontech laboratories, Inc. Mountain View, California, USA). ..

    Article Title: OsMYB99 Modulates Grain Wax Biosynthesis and Redox Homeostasis to Control Head Rice Yield and Chalkiness.
    Article Snippet: .. Full- length OsMYB99 CDS and truncations (OsMYB991- 117aa, OsMYB991- 160aa and OsMYB991- 210aa) were PCR- amplified and ligated downstream to the GAL4 DNA binding domain in pGBKT7 vector (Clontech, USA) by homologous recombination. .. Constructs and empty vector control (BD) were transformed into the yeast strain Y2HGold and plated on synthetic dropout medium without tryptophan (SD/- T).

    Polymerase Chain Reaction:

    Article Title: A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla
    Article Snippet: After incubation for 24 h, the transformed onion epidermal cells were stained with DAPI (100 ng/mL) and visualized under an inverted fluorescence microscope (Zeiss, Jena, Germany). .. The complete and various truncated versions of the CDS of BpbHLH1 were PCR amplified and fused in‐frame to the GAL4 DNA‐binding domain in the pGBKT7 vector to generate the pGBKT7‐ BpbHLH1 construct (Clontech laboratories, Inc. Mountain View, California, USA). ..

    Article Title: OsMYB99 Modulates Grain Wax Biosynthesis and Redox Homeostasis to Control Head Rice Yield and Chalkiness.
    Article Snippet: .. Full- length OsMYB99 CDS and truncations (OsMYB991- 117aa, OsMYB991- 160aa and OsMYB991- 210aa) were PCR- amplified and ligated downstream to the GAL4 DNA binding domain in pGBKT7 vector (Clontech, USA) by homologous recombination. .. Constructs and empty vector control (BD) were transformed into the yeast strain Y2HGold and plated on synthetic dropout medium without tryptophan (SD/- T).

    Construct:

    Article Title: A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla
    Article Snippet: After incubation for 24 h, the transformed onion epidermal cells were stained with DAPI (100 ng/mL) and visualized under an inverted fluorescence microscope (Zeiss, Jena, Germany). .. The complete and various truncated versions of the CDS of BpbHLH1 were PCR amplified and fused in‐frame to the GAL4 DNA‐binding domain in the pGBKT7 vector to generate the pGBKT7‐ BpbHLH1 construct (Clontech laboratories, Inc. Mountain View, California, USA). ..

    Transformation Assay:

    Article Title: Overexpression of PpGL2 from Prunus persica enhanced soybean drought tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Article Title: Overexpression of PpGL2 from Prunus persica Enhanced Soybean Drought Tolerance
    Article Snippet: .. For transcriptional activation assays, the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold. .. The pGBKT7 vector served as the negative control, while BD-p53 and AD-Sv40 large T antigen served as positive control.

    Clone Assay:

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers
    Article Snippet: Full‐length open reading frames (ORFs) of TrAP were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGADT7‐AD (Takara), fusing them with the Gal4 activator domain. .. Full‐length ORFs of REn were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGBKT7‐BD, fusing with the Gal4 DNA‐binding domain (Takara: 630443). .. Full‐length ORFs of Argonaute 1 (AGO1), regulator of gene silencing calmodulin (rgs‐CaM) and autophagy‐related gene (ATG7) were obtained from plant cDNA and cloned into pGBKT7‐BD (Takara).

    Homologous Recombination:

    Article Title: OsMYB99 Modulates Grain Wax Biosynthesis and Redox Homeostasis to Control Head Rice Yield and Chalkiness.
    Article Snippet: .. Full- length OsMYB99 CDS and truncations (OsMYB991- 117aa, OsMYB991- 160aa and OsMYB991- 210aa) were PCR- amplified and ligated downstream to the GAL4 DNA binding domain in pGBKT7 vector (Clontech, USA) by homologous recombination. .. Constructs and empty vector control (BD) were transformed into the yeast strain Y2HGold and plated on synthetic dropout medium without tryptophan (SD/- T).



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    Image Search Results


    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Journal: Molecular Plant Pathology

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers

    doi: 10.1111/mpp.70202

    Figure Lengend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Article Snippet: Full‐length ORFs of REn were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGBKT7‐BD, fusing with the Gal4 DNA‐binding domain (Takara: 630443).

    Techniques: Expressing, Binding Assay, Selection, Plasmid Preparation